Agarose Multimodal Ion Exchange Media
INQUIRY
In the realm of biotechnology and biochemistry, the separation and purification of biomolecules are critical processes. Among the various techniques employed, ion exchange chromatography stands out for its efficiency and versatility. Traditional ion exchange media typically rely on single-mode interactions—either cationic or anionic. Unlike traditional ion exchange media, multimodal ion exchange media combine ionic interactions with other binding mechanisms, such as hydrophobic, hydrogen bonding, and van der Waals interactions. This multifaceted approach enhances the media's ability to differentiate between biomolecules, leading to improved separation performance.
Alfa Chemistry is dedicated to providing you with high-quality agarose multimodal ion exchange media. They are chromatography media with hydrophobic and ionic groups bonded to highly cross-linked agarose microspheres, exhibiting selectivity different from that of traditional ion exchange chromatography media.
Our Products
| Product Name | Agarose 90HCM | Agarose 90HAM |
| Catalog Number | ACIR-IEC016 | ACIR-IEC017 |
| Media Type | Multimodal | Multimodal |
| Matrix | Cross-linked agarose | Cross-linked agarose |
| Particle Size | 90 µm | 90 µm |
| Ligand | Multimodal weak cation with phenyl | Multimodal weak anion with phenyl |
| Ligand Density | ~0.12 meq/ml | ~0.10 meq/ml |
| Dynamic Binding Capacity | ~50 mg Lysozyme/ml | ~30 mg BSA/ml |
| Maximum Pressure | 0.3 MPa | 0.3 MPa |
| Clean In Place | 1 M NaOH | 1 M NaOH |
| Recommended Flow Velocity | 150-250 cm/h | 150-250 cm/h |
| pH Stability | 2-12 | 2-12 |
| Chemical Stability | Stable to commonly used buffers: 1 M HAc, 1 M NaOH, 1 M HCl, 70% ethanol, 30% isopropyl alcohol, 30% acetonitrile,1% SDS, 6 M guanidine hydrochloride, 8 M urea, etc. Avoid prolonged contact with strong oxidizing agents. | Stable to commonly used buffers: 1 M HAc, 1 M NaOH, 1 M HCl, 70% ethanol, 30% isopropyl alcohol, 30% acetonitrile,1% SDS, 6 M guanidine hydrochloride, 8 M urea, etc. Avoid prolonged contact with strong oxidizing agents. |
| Operational Temperature | 4-30 °C | 4-30 °C |
| Storage Conditions | 4 to 25 °C, 20% Ethanol | 4 to 25 °C, 20% Ethanol |
*If the specifications of our products do not meet your requirements, we can also provide you with customized services.
Product Characteristics
The combination of multiple interaction mechanisms allows for finer discrimination between molecules with similar charge properties but different structural or hydrophobic characteristics. This selectivity is particularly beneficial in the separation of complex protein mixtures or the purification of monoclonal antibodies.
- Increased Binding Capacity
Multimodal media often exhibit higher binding capacities compared to their single-mode counterparts. This is due to the synergistic effect of the multiple interaction sites, which can capture and hold a greater amount of target molecules.
Agarose multimodal ion exchange media are designed to perform under a wide range of conditions, including varying pH and ionic strengths. This robustness makes them suitable for different stages of the purification process, from capture to polishing.
At Alfa Chemistry, our commitment to excellence extends beyond product quality. We also offer unparalleled customer support and technical expertise to ensure you get the most out of our agarose multimodal ion exchange media. Our team of experts is always available to assist with product selection, application advice, and troubleshooting, ensuring that you have the support you need to achieve your goals. If you have any need, please contact us immediately. We look forward to working with you.
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