Chromatographic Media List
The chromatographic media refers to the filler resin in the chromatographic column, most of which are styrene-divinylbenzene copolymers. There is a big difference between the ion exchange functional groups and capacity of these filler resins, which usually include three forms of high-performance ion exchange chromatography (HPIC), ion exclusion chromatography (HPIEC) and ion pair chromatography (MPIC). Among them, high-performance ion exchange chromatography (HPIC) uses low-capacity ion exchange resins, ion exclusion chromatography (HPIEC) uses high-capacity resins, and ion pair chromatography (MPIC) uses porous resins that do not contain ion exchange groups. In addition, the separation principles of the three types of separation methods are basically quite different. The separation principle of high performance ion exchange chromatography (HPIC) is ion exchange; the separation principle of ion exclusion chromatography (HPIEC) is ion exclusion; the separation principle of ion pair chromatography (MPIC) is based on adsorption and ion pair formation.
Ion exchange chromatography is an important downstream processing technology for biopolymers that is widely used commercially, especially in the production of biopharmaceuticals. Using chloromethylated polystyrene cross-linked microspheres (chlorine spheres) with high hardness and low price as the carrier matrix, N,N-dimethylformamide (DMF) as the solvent, and N,N-diisopropylethylamine (DIEA) catalyzed, Connect the synthesized Lys (Boc)-OEt and ethyl chloroacetate to the microspheres. After hydrolysis, the carboxyl group is exposed and chelated with Ni2+, and a new type of nickel ion affinity chromatography medium can be prepared. The medium preparation method is easy to operate, mild reaction conditions, and the raw materials used are cheap and easy to obtain. This metal ion affinity chromatography has selective affinity for short peptides containing histidine and can be used for the separation of short peptides containing histidine or cysteine. ( Lili XU, et al., 2016)
Affinity chromatography is a chromatographic technique that uses specific affinity between biomolecules for separation. For example, there is a specific affinity between enzymes and substrates and inhibitors, antibodies and antigens, hormones and receptors. Ligands prepare stationary phases for chromatographic separation or intermolecular interaction studies. Affinity chromatography with thrombin as ligand can be used for the screening of direct inhibitors of thrombin.( Wang X, et al., 2013)In short, as mentioned above, ionic resins are widely used in various industries as chromatographic media, especially the pharmaceutical industry.
- Glucose-fructose separation
- Polyol separation
- Nucleotide separation
- Separation of rare earth elements and various fission products
- Lili XU, et al. Preparation and properties of new nickel ion affinity chromatography medium[J]. Chinese Journal of Chromatography, 2016.
- Wang X, et al. Preparation of thrombin affinity chromatography media[J]. Chinese journal of chromatography, 2013.