High Rigidity Agarose Ion Exchange Media

High rigidity agarose ion exchange media are a class of chromatography resins used to purify biomolecules such as proteins, peptides and nucleic acids. Compared to fast flow agarose ion exchange media, these media have higher rigidity, faster mass transfer rates, and greater tolerance. Depending on the particle size, our products are subdivided into high capacity and high flow media (HF) series and high capacity, high flow and high-performance resolution media (HPR) series.

Our Products

High Capacity and High Flow Media (HF) Series

High Capacity, High Flow and High-Performance Resolution Media (HPR) Series

Product Name	Agarose SP HF	Agarose CM HF	Agarose DEAE HF	Agarose Q HF
Catalog Number	ACIR-IEC001	ACIR-IEC002	ACIR-IEC003	ACIR-IEC004
Matrix	High rigidity agarose
Ligand	Sulphopropyl	Carboxymethyl	Diethylaminoethyl	Quaternary ammonium
Ion Exchanger	Strong cation exchanger	Weak cation exchanger	Weak anion exchanger	Strong anion exchanger
Particle Size Range	45-165 µm
Average Particle Size	90 µm
Ionic Capacity	140-200 µmol (H+)/ml	90-120 µmol (H+)/ml	290-350 µmol (Cl-)/ml	160-220 µmol (Cl-)/ml
pH Stability	4-12 (long term), 3-14 (short term)		2-12 (long term), 2-14 (short term)
Maximum Flow Velocity	1000 cm/h
Operating Pressure	≤0.5 MPa
Chemical Stability	Stable to all commonly used buffers: 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol. Avoid using oxidizing agents, cationic detergents, and cationic buffers.		Stable to all commonly used buffers: 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol. Avoid using oxidizing agents, anionic detergents, and anionic buffers.
Storage Conditions	4 to 30 °C, 0.2 M NaAc, 20% Ethanol	4 to 30 °C, 20% Ethanol	4 to 30 °C, 20% Ethanol
Application	This series of products are suitable for the separation and purification of all charged biological molecules such as proteins, peptides and nucleic acids.

*If the specifications of our products do not meet your requirements, we can also provide you with customized services.

Product Characteristics

• High pressure resistance, ultra-high flow rates, and increased productivity.
• Fast mass transfer.
• Excellent compatibility, suitable for initial capture or purification of biomolecules of various sizes.
• The purification process is flexible and can be combined with hydrophobic chromatography.

Product Name	Agarose SP HPR	Agarose DEAE HPR	Agarose Q HPR
Catalog Number	ACIR-IEC005	ACIR-IEC006	ACIR-IEC007
Matrix	Highly cross-linked agarose and cellulose	Highly rigid agarose	Highly cross-linked agarose and cellulose
Ligand	Sulphopropyl	Diethylaminoethyl	Quaternary ammonium
Ion Exchanger	Strong cation exchanger	Weak anion exchanger	Strong anion exchanger
Particle Size Range	25-45 µm
Average Particle Size	37 µm
Ionic Capacity	180-280 µmol (H+)/ml	110-160 µmol (Cl-)/ml	180-250 µmol (Cl-)/ml
Binding Capacity	100 mg Lysozyme/ml media	-	120 mg BSA/ml media
pH Stability	3-14 (short term), 4-12 (working), 4-12 (long term)	2-14 (short term), 2-12 (long term)	2-14 (short term), 2-12 (working), 2-12 (long term)
Maximum Flow Rate	300-400 cm/h (0.4 MPa)	400 cm/h	300-400 cm/h (0.4MPa)
Operating Pressure	≤0.5 MPa
Chemical Stability	Stable to all commonly used buffers: 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol. Avoid using oxidizing agents, cationic detergents, and cationic buffers.	Stable to all commonly used buffers: 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol. Avoid using oxidizing agents, anionic detergents, and anionic buffers.
Storage Conditions	4 to 30 °C, 0.2 M NaAc, 20% Ethanol	4 to 30 °C, 20% Ethanol
Application	This series of products are suitable for the separation and purification of all charged biological molecules such as proteins, peptides and nucleic acids.

*If the specifications of our products do not meet your requirements, we can also provide you with customized services.

Product Characteristics

• Fast, simple and convenient.
• High pressure resistance, high resolution.
• Wide range of applications.
• Highly flexible purification process.

If you are interested in our products, please feel free to contact us. Whether you need detailed product information, technical advice, or customized solutions, Alfa Chemistry will do its best to help you.