Biogenic amines are mainly formed by decarboxylation of amino acids or amination of aldehydes and ketones, and have certain biological activity and low relative molecular mass. Alfa Chemistry can help you determine various types of biogenic amines. Our ion chromatography is not only fast and accurate, but also simple and efficient.
Introduction
Biogenic amines are widely found in aquatic products, meat, fruits, beverages and other foods. As a normal physiological component of the human body, biogenic amines play an important physiological regulation function, but excessive intake can also produce certain toxic effects. Commonly used detection methods for biogenic amines include gas chromatography, liquid chromatography, ion chromatography and so on. The pre-treatment steps of gas chromatography are cumbersome, requiring purification and concentration, solid phase extraction, etc. However, liquid chromatography is time-consuming, easy to introduce by-products, and low efficiency. Compared with the first two methods, ion chromatography has the advantages of fast, accurate and sensitive.
What We Offer
- Equipment
Ion chromatograph (equipped with conductivity detector and autosampler), pure water meter, various biological amine standards (diethanolamine, butylamine, spermidine, spermine, etc.).
- Selection of elution method
There are often multiple biogenic amines in the sample at the same time, and the affinity of various biogenic amines to the stationary phase of the chromatographic column is different. Therefore, it is necessary to select the elution method. Common elution methods include isocratic elution, gradient elution, etc., which need to be selected and optimized based on the actual situation.
- Detection Steps
1. Take an appropriate amount of sample, add deionized water, shake it, and let it stand for a period.
2. Adjust the pH value of the solution to 3～5, transfer to a volumetric flask to make the volume constant, shake well and set aside.
3. Take an appropriate amount of the solution and filter it with a water-phase filter, and then pass it through a pre-activated RP column (pass through methanol, deionized water in turn, and let it stand). Discard the first few milliliters of solution, collect the clear liquid, and detect with an ion chromatograph.
- Interference test
Some small molecule cations (such as sodium ions, calcium ions, magnesium ions, etc.) in the sample will interfere with the measurement, so an interference test is needed to eliminate the influence of these cations.
Alfa Chemistry has advanced ion chromatography equipment. Our goal is to help customers solve their bio-amine detection projects. We can provide one-stop testing services, the mission must be achieved. Our customers can directly contact our experts and provide timely feedback on any online inquiries. If you are interested in our services, please contact us.
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