Separation & Purification of Peptide by Ion Chromatography

Separation & Purification of Peptide by Ion Chromatography

INQUIRY

Alfa Chemistry provides professional peptide separation and purification services. The peptide product obtained by the chemical synthesis method is not high in purity and is a crude product. The presence of impurities will affect the efficacy of peptide drugs, so peptides need to be separated and purified. We can provide peptide separation and purification services ranging from milligrams to hundreds of kilograms, with high efficiency, high quality and reasonable price.

Introduction

Separation and Purification of Peptide by Ion Chromatography

In recent years, the progress of chemical synthesis technology has increased the discovery and synthesis speed of new active peptides. Peptide drugs are gradually becoming an important branch of medicinal chemistry. It is worth noting that various side reactions, racemization and other side reaction peptides will occur in the process of chemical synthesis of peptides. In addition, during the deprotection process, due to the residue of the protecting group, the cleavage of the peptide bond, alkylation, etc. will also cause impurities. The molecular structure of many impurities is very similar to that of synthetic peptides, only a certain group on the side chain of a certain amino acid residue is different, showing similar properties to the product. This brings difficulties to the separation and purification of peptides. Therefore, according to the requirements of the target peptide, the purification method and process are extremely important.

Ion exchange chromatography achieves separation through the charge interaction between the charged solute molecules and the exchangeable ions in the ion exchanger. The small difference in charge in the charged molecules can be separated, and it has a higher separation capacity. Since almost all biological macromolecules are polar and can be charged, the ion exchange method has been widely used in the separation and purification of biological macromolecules. Ion exchange chromatography with high resolution, large working capacity and easy operation can be used as an important method for peptide separation and purification.

Influencing factors

The choice of ion exchanger is an important factor affecting the separation and purification results of peptides. The type of ion exchanger and the strength of the exchange functional group will affect the separation and purification results. Ion exchange columns can be divided into two categories: cation columns and anion columns. There are also some new types of resins, such as macroporous resins, homogeneous resins, ion exchange cellulose, dextran gels, and agarose gel resins. Secondly, the applicable pH value and elution conditions should be further determined, especially the ionic strength and salt concentration of the eluent. Generally, the elution method of polypeptide is concentration gradient elution, which is used to change the concentration of sodium chloride or potassium chloride in the mobile phase to achieve the purpose of elution.

Ion exchange chromatography has become an important separation method in peptide chemistry. As an expert in the ionic resin industry, Alfa Chemistry will provide you with the industry's top peptide separation and purification services. Our customers can directly contact our experts and provide timely feedback on any online inquiries. If you are interested in our services, please contact us.

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